Team Agreement

Members: Angela May, Noah Millner, and Eli Cox-Skall

Objective: We are testing 3 different types of grass growing in portland, sequencing them to find out what types of grasses they are

Schedule:

3/22/13 Wed- Noah and Eli worked together and stayed through lunch to complete the first stages of the experiment.

Note: Angela was absent on a field trip for Film&Lit

3/24/13 Fri- Angela Eli & Noah did sequencing together. Eli took pictures for the blog during experiment.

3/29/13 Wed- We loaded our samples into the gel using the micropipet.

Jobs Divided:

Eli

• Uploads and adds/ labels pictures on blog
• Sample info, methods
  writes out team agreement.

Angela

• Type team agreement
• Experimental design with noah

Noah

• Experimental design with angela
• Results & Analysis

where we collected our invasive grass from (Eli's backyard)

                   

invasive grass close up

where seeded grass was collected from

pre-gown grass (saud)

above and below are pictures of our 3 difrent spiecies removed from the ground

grinding the sample so that we are able to remove DNA from

condensing sample into tube and grind into soloution.

sample waiting

noah adds RNAse to sample.

samples above and below

this holds are three samples and incubates them in water for thirty minutes. (incubate at 37 degrees celcius)

inside the incubator our sample waits.

we wash out our mortal and pastel. 

we allow our sample to sit on 

we put in the cenerfuge for four minutes and two minutes.

vortexed to mix it.

Removing the supernatant from the test tube.

making sure not to cross contaminate the samples we used different tips for each sample and step. 

allowed our sample to incubate and amplifiy in thermal cycler.

the basic lab kit we used to do the majority of our experiment.  this included a tube holder, tubes, micro pipet and tips, graduayed cylinder.

the rest of these pictures are excess and giving an explanation would only be repetitive. 

TEAM AGREEMENT